Does anyone's car have issues with the glass vibrating and making noise while driving? Occasionally, there's a vibrating sound from the glass. Does anyone know what might be causing this?

You can search online, the new GX550 shakes a lot, typical Toyota. My CHR's glass also shakes badly when going over bumpy roads. I even put some 3M tape in the glass groove to make it slightly tighter. It's only a bit better when the window is half down or fully lowered.

My driver and passenger windows are making strange noises, and when I roll them up to the top, the motor makes a humming sound. 😊

There might be something loose with your window if it's not shaking.